保健酒中总黄酮测定方法的比选 金红 酿酒科技 2009年2 p79-81 关键词 保健酒、总黄酮、分光光度法

'~~~I,.Ri+2,10r:Jf.~2,5%..;fL2,~IJ1:.2(I.tt*~"t£I~f4?¥:ijJfJi:J9f.i=tm~fH730000;2.~~m~1i1l&1i"1f0j"i].tt*~~736201)m~:~t4t7iJl1J;t1*-flVI1i"f,t.-jifil."J1-i€0~#~i!-0~i!--I"A;tTJJ~,I!KJt"..,•/j/:lt4g-3E..,•/j/:lt1,lJJJi&.~*,J,-ff-iJf..*500nm~iJ1IJ;t.~i!-"::"I"A360nm~jL.g.ij!IJ;t1*-1Jtil1i"f-jifil."J€01-i.~",W;Jfj..•,/j/:ltigi&.~o~*k-Il}j•~i!-"::"€0RSD:h2.61%.~it-T~i!--€0RSD(2.79%);~i!-.::...9taJ.ti*j,.t*1tis11t,rt*or-to~.laJ:1*-1Jtil1i;,t.-jifI!J"];~JtJtJ.ti!-!:p1ll,H~~TS262.91:;TS261.4;TS261.7;0657.33tif«tFiRW:B3t~t.li8~:1001-9286(2009)02-0079-03ComparisonandSelectionoftheDeterminationMethodsofTotalFlavonoidsContentinHealthcareWineJINHongl,DUANHui2,HEYa-dun2,ZHANGLFandLIUFee(1.LightlndustryScientificResearchInstituteofGansuProvince,Lanzhou,Gansu730000;2.DunhuangWineIndustryCo.Ltd.•Dunhuang,Gansu73620I,China)Abstract:Thetwodeterminationmethodsoftotalflavonoidscontentinhealthcarewinewerecompared.MethodoneusedrutinasthecontrastsampleandtherevealingagentwasAl(NO})}andthedetectionwasoperatedat500nmwavelength.Methodtwocouldbeusedtomeasuretotalflavonoidscontentdirectlyat360nmwavelengthwithouttheuseofAl(NO})}.ThemeasurementresultsshowedthatRSDofmethodtwo(2.61%)wasbetterthanRSDofmethodone(2.79%)andmethodtwohadtheadvantagesincludinghighsensitivity,simpleoperationandreliablemeasurementresults.Keywords:healthcarewine;totalflavonoids;spectrophotometry~.~A~.~~¥M~~m~~#~~mtt,.rom~~%~~nA~~~~ttmfitt~m~~m,*.1.2i;1f,J~~~~~~~~~~~~*~~~,-®~.M~~pTfiF("ff£:AC,1¥J*i":l!JTjt-z,,~~jt*~~?JT;~/~Hx5!li~fJt.1-t~mJJt1~~~,If%~~~-=f,1*tftEj3rw:AC,J(7fm~*ID1-t~iiJfIJ~~&0~;SI[1i~!MHI*J:DNA;1llJtHl;1:J;*3UIHi8(LDL).1-t~Hlf!mi~~~*~AC,gg~1-t~iiJfIJr;1i~Dfg:AC,J(7fm~*ID1-t~~mo~~,~~~.M~~~~~~m~~m~~~iiJflJ~~&0~;~~~fIIi:AC,:J:OOE1m~)U)(1-tI~~&0~B~~~~g~~~£~~~~~~~~AZ-o~~;*:AC,J(7fm~WID1-t~iiJflJ;(;f1l&0~;~mt~:~1*.m~~.~~*.,m~.~••%~~~~u30~60§,L#lJ~~1-t~iiJfIj~~&0~;~_H~~jJnJ:m*~~~o*x*moo%~£~£~~~~~~E0~_H~nJ2Ik~~&jfHf:0~0~~1*.~£~~.~~~~~#~~*fi~m,Mffi1.3~~~*~~~~-#~~~~a0~1*.m~~.M*.~1.3.1~~-Wl~~~~o~*$7t~.M7t-=f~~*~~=M~¥,~~7t-=f:(£NaN02~m~,f"~~=!!i!l~f~,N:(£~"I1:~f4=1M*4~1r5~~,~AP+%~ttfS~il%~,:(£-~7Jf*~~:m:*I1&1&1.11)(.3-*~tif,gBt,ilH"TbtfS7ttIT,791~~~*.o722S7tjtjtJJtH:L#lJfR*f4~f)(~~1l&0~;Jt:,1.3.1.1f:;~maJ:l~~$iJ~~T:)AU§::It*f41t7j(~1)(~~~&0~;~~tl"U1ifr7j(mfR***J&:(£120·C~ffT~~tflm~pTxtff~£~::It*~1t7j(~1)(~~~&0~Jt:~*:L#lJff{.$tf4~21mgT/J~;fF~,J&~:I:Ej3rwmfm,~~~100mL.f)(~i~*~~&0~DT-I00A7t:fJTJ(1JL::It*~mJ(1JLmL~,~?£¥~100mL1*~,&P~~(4ij:1mL~*7CJj(p1&mE3WJ:2008-10-13i1=~urft:~h(1968-).Y:,Il1Hfi,$±o 80I!iIUI1l#-tt2009:¥~2M(.~,~176M)"LIQUOR-MAKINGSCIENCE&TECHNOLOGY2009No.2(Tol.I76)T0.21mg):P"Tm~o*-11J5*-@]~9:~jy!IJjE~*(n=6)71"~IJ#HJti:i!:~f~i"1E:P"Tm~0mL,1.0mL,2.0mL,1JI:lAfff-(iP"T:S:(fig)@]45cP"T(fig)@]45c~(%)3.0mLAOmL,5.0mLfU6.0mL,71"~fJj[r25mLtt~3.941198.464.00273.907497.62"If"=P,~1107.](~6mL,m1100.7mLiL8-9SIffJ1HI&fl*Jm~3.941198.461.0mL,Ylt~J,jj!{j[6min,nO0.5mLiL8-9M~fgm~4.1604103.941.0mL,t*~,1i!{j[6min,nO1mollL8-9~¥C1tfl*JiJt~4.1772104.3610.0mL,~n07.](~~IJoc,~~,1i!{"lt15min,fflIemtt4.1604103.94~lIII.,:ft71":7"t:7"tJJtit8-9500nmyEz*~7li!JEIJP}k:7"tJJt,~~4.0423100.9911&3.907497.62:)"tJJt7g~~f~,1:&JJt7g:m~f~,1~tI:lf~i"1EE!E~Er"1@JY33.890697.20JrWo~"I1:@JY3JrW7gy=0.458x-0.0048,;fILk*~4.1100102.68r=0.99970f~f{EBtl~mOO104.0928102.25<"3O.74.0759101.83SiF(f£pT:1l(mg)IE1t&PT(fig)[i=ilt&~(%)ffJ¥,~2:ctiY!lJilt,2:B~~~ffl2.0mL~"llMt~ff2l.0mL3.914297.79~.t&*~!J&.~i"i"1J04.00273.895797.332.27i"it--=:..if.]1;1UJcA3.904397.54~~=3.895797.33rY!~1J~j!ij1,*iiJffl~j!ij~1ii"JllMmffJ¥J§",1it~79J1J4.00273.917197.86~~Il.&:7"t~,79J1J~~!/&.~~M~.~"jf£o3.972999.26{g~~1±~JE:CD*~~*fflI!&Jfil~~1.0mLT4.0330100.76.~JI[,1"tJ§110A1g!U1Jtmc~*1iIl.&41(,{g~,~$*4.0027396869915396059895~:f$~N~•••mc~l!&ffl,rm~ffl;g~.~JI[~*3.9772993600,xt!/&.~~Qr"J;®~ll.&fflT~~~••mc~~A~4.00274.123110301mtt~,~$*••mc~.~.M~~mtt~*oo,~4.118810290~*m~~*;®~~~~~.m••mc~.~~.4.1231103.Dl4.00274110310269ffll,I:!P§!J&~moo;ffl;at¥5Jitll.BtfllfflEj3MtJEJillBt.8"1mt:Ii041317103.22@*~~N~~~ffl"llB~Jill.~.~m~X~~~,~384859615:ctiJt~£~,~~~~T.m~.~~~:f$£.iJt~e4.00273848596153.84859615.~i"i~,.~"jf£~1~M,{g~,xtT~j!ijIiiJ~:I:~~~:f$£79JIJiJt8"1e.iIfU{~,~:ct*f.JT%il&~~ilt~,tm*~£EB*2PIH.ill,*29=SJ9-¥::5lIJD1&-¥~99.37%,~~j!ijIiiJ~}I~i"i"1JB,t25mL~"llM;tR*N~tE••mc~~~m~~2.60,RSD~2.61%09=Jll.&ffl~~m*~~Jill.%,~~~~~Jill."llM~ffl}l,~¥Ij¥5tJill.~*~"llM~7G~Bt,fI}~~,~j)11j,e.~i!R2~m~~*JT£~*oEBT~••9=J~m*~~~»~}I~$.tt,~~~~~*~~~ft~~~**~£~,*~~*ffl~#3~~~~~~.~~~~••9=J~~m~~.lttfi.~*~~-~?T~xtP.~£,1iJ1j~fg-5Jlf.1ifnHi*J~~~tFr,3f·ltt1iTIID41(-¥~ilt~,*fHI*~~~i5*,~#iY!IJ~~JfIJ~~~,500om:Qt79JIJ~~.t@9=~J,g,~j!ijIiiJ~::I:,!ij~~4}~5f1j!l$0~=tE~*360om!It1it~79JIJ~~••9=J~m~~iI:,2.1jn}-if.]1JCMc.~,~m:ffl1il!j~f.g~~,~~~*t~~,~~=~RSD~~~-.~~e.~~M~.~tt,m~~~~2.61%~f7tTj]it-!-~RSD2.79%,~~=RfW:JJt~,IL*J.m~:CD~xt~i~ltt1i)lU"~:ctW9=J,ffl"llMxt~f*f1::fijiI,~*PI:30tt*m.~~~£lttfi"llMmM:ctW9=J,~*N~ffl"ll~~:X:~:I:ltt1i~¥@JiJfM,$7X~m,79J1J~ill*~e••~"jf£~[1]xJ"GS[:(*fit~£J)]~nlt:5tt1iLi9N1f7t:[M]jt*:9="OO$£I~ili~;®~*1ill.&:7"tJjt~iY!IJ~:ctif.9~=,J~m*~~~"jf£~JtlH±,2002.159-160.itj:~,!ijAP+%$;~~Il~Jitir!jWj"jf£J1lJ:~.~,Ilif!;gBt18]~m.~~~.~,[2]9:li~AB!;,!HIlOO~!Jl!.~91~.9="*AB!;jt;fl:lOO~!Jl!.[M]jt1l.&:7"t~~.~~~~10~n~%/"]1:{:t~I~iliJ:&t±,2005.(+-+-+-+-+-+-+-+-+-+-+-+-~-+-+-+-+-+-+-+-+-+~-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-)f+~5S,ft:r~A.fr1t-~-~j~++;$;fIJtfl.:2008.if-,~;1]..4-iarillir~JJi!.1~itJ!tA37.41[,JC.,~~t.JiC*52%;A4;fjLJ!t9.131["~~t.JiC*48%0.i.~~4J-~tAitt.A+~l!lEJiMT~~l1l"1{i,Y:J.~1Jt€r"JJI!p-:Y;i~AH~~-;tJ"4.o+i2006.if-,4-i"arillir1~%J!tA~jlJ141[,JC.;2007.if-,1~itJ!tA"R.oj:241[,JC.;2008.if-,1~itJ!tA"R.oj:371[,JC.03.if-fa],4-iarilJir~trJJl!r.,t:k:it"f##<£.Jl,~JJi!.r£.Jl"~M.ff)t"0t!lfrlit~at,~;1]..4-iarilJir€r"Jif..foJi*jjt~~~a11~itJ!tA~"R.oj:24.251[,JC.,~~t.JiC*68.5%,tftJiJl;~;1]..tf~ftif,EJilJiJjrJh~-,#-t!~j:l!l~..I.~,t·%JiA~J!JH~J1l.if-.$.£W"tfl!lllt!!.-£~#",~.A~r1~tfl!l~1~"i4JiJAJ1l~A;f,t€r"J"tfOO~~~1~·tr;Jj,f,if~"0(1111)J.~-+--+---+---+---+--+---+---+---+---+---+----+---+---+---+---+---+----+--+---+---+---+--+---+---+---+---+---+---+--_+_--+---+---+-..-+-..-+---+---+---+---+----+---t---t----+---+--+---+---+---+-..'